Projects per year
Abstract
Current optical microscope objectives of low magnification have low numerical aperture and therefore have too little depth resolution and discrimination to perform well in confocal and nonlinear microscopy. This is a serious limitation in important areas, including the phenotypic screening of human genes in transgenic mice by study of embryos undergoing advanced organogenesis. We have built an optical lens system for 3D imaging of objects up to 6 mm wide and 3 mm thick with depth resolution of only a few microns instead of the tens of microns currently attained, allowing sub-cellular detail to be resolved throughout the volume. We present this lens, called the Mesolens, with performance data and images from biological specimens including confocal images of whole fixed and intact fluorescently-stained 12.5-day old mouse embryos.
Original language | English |
---|---|
Article number | e18659 |
Number of pages | 15 |
Journal | eLife |
Volume | 5 |
DOIs | |
Publication status | Published - 23 Sep 2016 |
Keywords
- microscope
- embryos
- sub-cellular resolution
- optical lens
- 3D imaging
- biological specimens
Profiles
Projects
- 1 Finished
-
MESOLAB: A Centre For Optical Mesoscopy For Biomedical Research At The University Of Strathclyde
McConnell, G. & Dempster, J.
MRC (Medical Research Council)
13/02/13 → 12/02/20
Project: Research
Datasets
-
A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout
McConnell, G. (Creator), Tragardh, J. (Creator), Amor, R. (Creator), Reid, E. (Creator), Dempster, J. (Creator) & Amos, W. (Creator), University of Strathclyde, 1 Sep 2016
DOI: 10.15129/36076e72-05e5-4f15-9cd2-0983de382b0d
Dataset
Research output
- 1 Article
-
Fast optical sectioning for widefield fluorescence mesoscopy with the mesolens based on HiLo microscopy
Schniete, J., Franssen, A., Dempster, J., Bushell, T. J., Amos, W. B. & McConnell, G., 2 Nov 2018, In: Scientific Reports. 8, 10 p., 16259.Research output: Contribution to journal › Article › peer-review
Open AccessFile3 Citations (Scopus)16 Downloads (Pure)