Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging

Stephen D. Grant; Gemma S. Cairns; Jordan Wistuba; Brian R. Patton

doi:10.12688/f1000research.21294.1

Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging

Stephen D. Grant, Gemma S. Cairns, Jordan Wistuba, Brian R. Patton

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

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Abstract

We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the SuperResolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.

Original language	English
Number of pages	7
Journal	F1000Research
Early online date	26 Nov 2019
DOIs	https://doi.org/10.12688/f1000research.21294.1
Publication status	E-pub ahead of print - 26 Nov 2019

Keywords

light microscopy
super-resolution
3D printing

Access to Document

10.12688/f1000research.21294.1Licence: CC BY 4.0

Grant-etal-F1000-2019-Adapting-the-3D-printed-Openflexure-microscope-enables-computational-super-resolution-imagingFinal published version, 897 KBLicence: CC BY 4.0

Cite this

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title = "Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging",

abstract = "We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the SuperResolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.",

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KW - super-resolution

KW - 3D printing

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Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging

Abstract

Keywords

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Other files and links

Low-cost, open-access imaging for identifying and quantifying water quality (GCRF)

Multiscale neural imaging - from synapse to whole-organism

Super-resolution microscopy for clinical research: Detecting mitochondrial interactions with intracellular bacteria after bacterial challenge.

Data for: "Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging"

Cite this

Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging

Abstract

Keywords

Access to Document

Other files and links

Projects

Low-cost, open-access imaging for identifying and quantifying water quality (GCRF)

Multiscale neural imaging - from synapse to whole-organism

Super-resolution microscopy for clinical research: Detecting mitochondrial interactions with intracellular bacteria after bacterial challenge.

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Data for: "Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging"

Cite this