Abstract
A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-based microgel constitutes living materials that control stem cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III7-10 fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow–derived mesenchymal stem cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties.
Original language | English |
---|---|
Article number | 100011 |
Number of pages | 9 |
Journal | Materials Today Bio |
Volume | 2 |
Early online date | 18 Jun 2019 |
DOIs | |
Publication status | E-pub ahead of print - 18 Jun 2019 |
Keywords
- droplet-based microfluidics
- bioprinting
- engineered bacteria
- cell engineering
- living materials
- stem cells