Breath and plasma metabolomics to assess inflammation in acute stroke

Waqar Ahmed, Iain R. White, Maxim Wilkinson, Craig F. Johnson, Nicholas Rattray, Amit K. Kishore, Royston Goodacre, Craig J. Smith, Stephen J. Fowler

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Inflammation is strongly implicated in both injury and repair processes occurring after stroke. In this exploratory study we assessed the feasibility of repeated sampling of exhaled volatile organic compounds and performed an untargeted metabolomic analysis of plasma collected at multiple time periods after stroke. Metabolic profiles were compared with the time course of the inflammatory markers C-reactive protein (CRP) and interleukin-6 (IL-6). Serial breath sampling was well-tolerated by all patients and the measurement appears feasible in this group. We found that exhaled decanal tracks CRP and IL-6 levels post-stroke and correlates with several metabolic pathways associated with a post-stroke inflammatory response. This suggests that measurement of breath and blood metabolites could facilitate development of novel therapeutic and diagnostic strategies. Results are discussed in relation to the utility of breath analysis in stroke care, such as in monitoring recovery and complications including stroke associated infection.

Original languageEnglish
Article number21949
Number of pages14
JournalScientific Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 9 Nov 2021

Keywords

  • stroke
  • plasma metabolomics
  • inflammation
  • acute stroke
  • therapeutic an diagnostic strategies
  • post-stroke inflammatory response

Cite this