TY - JOUR
T1 - Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry
AU - Denver, Nina
AU - Khan, Shazia
AU - Stasinopoulos, Ioannis
AU - Church, Colin
AU - Homer, Natalie Z.M.
AU - MacLean, Margaret R.
AU - Andrew, Ruth
PY - 2019/4/30
Y1 - 2019/4/30
N2 - Analysis of catechol estrogens (2 & 4 hydroxy-estrone and estradiol) has proven troublesome by liquid chromatography tandem mass spectrometry due to their low concentrations, short half-lives and temperature-labile nature. Derivatization to methyl piperazine analogues has been reported for a panel of 9 estrogens in, “Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry” (Denver et al., 2019). Data show alteration of the base catalyst in this method was required to allow detection of catechol estrogens to low levels. Data also highlight the challenges faced in chromatographic separation of isomers and isotopologues, which were partially overcome by employing an extended column length and reduced oven temperature. In addition, data analysis displayed significant matrix effects during quantitation in plasma, following solid-phase extraction, despite efficient recoveries.
AB - Analysis of catechol estrogens (2 & 4 hydroxy-estrone and estradiol) has proven troublesome by liquid chromatography tandem mass spectrometry due to their low concentrations, short half-lives and temperature-labile nature. Derivatization to methyl piperazine analogues has been reported for a panel of 9 estrogens in, “Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry” (Denver et al., 2019). Data show alteration of the base catalyst in this method was required to allow detection of catechol estrogens to low levels. Data also highlight the challenges faced in chromatographic separation of isomers and isotopologues, which were partially overcome by employing an extended column length and reduced oven temperature. In addition, data analysis displayed significant matrix effects during quantitation in plasma, following solid-phase extraction, despite efficient recoveries.
KW - catechol estrogen
KW - liquid chromatography
KW - mass spectrometry
KW - metabolites
UR - http://www.scopus.com/inward/record.url?scp=85063057333&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2019.103740
DO - 10.1016/j.dib.2019.103740
M3 - Article
AN - SCOPUS:85063057333
VL - 23
JO - Data in Brief
JF - Data in Brief
SN - 2352-3409
M1 - 103740
ER -