Full system considerations for multiphoton microscopy

D.L. Wokosin; J.M. Girkin; Periasamy Ammasi; Peter T.C. So

doi:10.1117/12.424558

Full system considerations for multiphoton microscopy

D.L. Wokosin, J.M. Girkin, Periasamy Ammasi (Editor), Peter T.C. So (Editor)

Institute Of Photonics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

A most-important-variables analysis of practical, successful multiphoton excitation fluorescence microscopy is presented. The key strength of multiphoton imaging -- localization of the excitation volume -- helps to decouple the excitation from the emission; the emission no longer needs to be imaged. The presentation starts with maximizing the detected signal and proceeds to the laser source considerations. The main goal is to match the instrumentation to the biological problem. The main aspects covered are... Emission collection: potential signal; Focal plane mismatch: chromatic aberration; Light detected: actual signal; Live cell imaging: preservation of biological function; Deep imaging: preservation of image contrast; Signal production: potential limitations.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences (Proceedings of SPIE Volume: 4262)
Pages	231-237
Number of pages	6
Volume	2
DOIs	https://doi.org/10.1117/12.424558
Publication status	Published - Apr 2001

Keywords

optics
photonics
multiphoton microscopy

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10.1117/12.424558

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title = "Full system considerations for multiphoton microscopy",

abstract = "A most-important-variables analysis of practical, successful multiphoton excitation fluorescence microscopy is presented. The key strength of multiphoton imaging -- localization of the excitation volume -- helps to decouple the excitation from the emission; the emission no longer needs to be imaged. The presentation starts with maximizing the detected signal and proceeds to the laser source considerations. The main goal is to match the instrumentation to the biological problem. The main aspects covered are... Emission collection: potential signal; Focal plane mismatch: chromatic aberration; Light detected: actual signal; Live cell imaging: preservation of biological function; Deep imaging: preservation of image contrast; Signal production: potential limitations.",

keywords = "optics, photonics, multiphoton microscopy",

author = "D.L. Wokosin and J.M. Girkin and Periasamy Ammasi and So, {Peter T.C.}",

year = "2001",

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doi = "10.1117/12.424558",

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Y1 - 2001/4

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AB - A most-important-variables analysis of practical, successful multiphoton excitation fluorescence microscopy is presented. The key strength of multiphoton imaging -- localization of the excitation volume -- helps to decouple the excitation from the emission; the emission no longer needs to be imaged. The presentation starts with maximizing the detected signal and proceeds to the laser source considerations. The main goal is to match the instrumentation to the biological problem. The main aspects covered are... Emission collection: potential signal; Focal plane mismatch: chromatic aberration; Light detected: actual signal; Live cell imaging: preservation of biological function; Deep imaging: preservation of image contrast; Signal production: potential limitations.

KW - optics

KW - photonics

KW - multiphoton microscopy

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U2 - 10.1117/12.424558

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M3 - Chapter

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SP - 231

EP - 237

BT - Multiphoton Microscopy in the Biomedical Sciences (Proceedings of SPIE Volume: 4262)

ER -

Full system considerations for multiphoton microscopy

Abstract

Keywords

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