Abstract
A newly developed hardware-friendly non-iterative fluorescence lifetime imaging (FLIM) analysis method was verified in an FPGA chip. Its performances were also demonstrated on two-photon FLIM images of gold nanorods (GNRs)-Cy5 labelled Hela cells. The results obtained by the proposed method can be presented in a polor plot to be compared to the widely used phasor (Phasor) approach. Combining our method with Phasor will be very useful in FLIM analysis.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Place of Publication | Bellingham, WA |
| Volume | 9536 |
| DOIs | |
| Publication status | Published - 2015 |
| Event | Advanced Microscopy Techniques IV; and Neurophotonics II - Munich, Germany Duration: 24 Jun 2015 → 25 Jun 2015 |
Conference
| Conference | Advanced Microscopy Techniques IV; and Neurophotonics II |
|---|---|
| Country/Territory | Germany |
| City | Munich |
| Period | 24/06/15 → 25/06/15 |
Keywords
- center-of-mass method
- field programmable gate array
- fluorescence lifetime imaging microscopy (FLIM)
- gold nanorods
- time-correlated single-photon counting
- time-resolved fluorescence imaging
- two-photon FLIM