Inhibitor of cysteine peptidase does not influence the development of Leishmania mexicana in Lutzomyia longipalpis

Lucie Jecna, Anna Svarovska, Sébastien Besteiro, Jeremy C. Mottram, Graham H. Coombs, Petr Volf

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4 Citations (Scopus)


It has been proposed that the natural cysteine peptidase inhibitor ICP of Leishmania mexicana protects the protozoan parasite from insect host proteolytic enzymes, thereby promoting survival. To test this hypothesis, L. mexicana mutants deficient in ICP were evaluated for their ability to develop in the sand fly Lutzomyia longipalpis. No significant differences were found between the wild-type parasites, two independently derived ICP-deficient mutants, or mutants overexpressing ICP; all lines developed similarly in the sand fly midgut and produced heavy late-stage infections. In addition, recombinant L. mexicana ICP did not inhibit peptidase activity of the midgut extracts in vitro. We conclude that ICP has no major role in promoting survival of L. mexicana in the vectorial part of its life cycle in L. longipalpis.
Original languageEnglish
Pages (from-to)605-609
Number of pages4
JournalJournal of Entomology
Issue number3
Publication statusPublished - 2009


  • sand fly
  • leishmania
  • phlebotomus
  • cysteine peptidase

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