Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

A Basso, R V Ulijn, S L Flitsch, G Margetts, I Brazendale, C Ebert, L De Martin, P Linda, S Verdelli, L Gardossi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


The application of biotransformations in solid phase synthesis is an attractive alternative to chemical methodologies. An important issue that needs to be addressed in this context is accessibility of functional groups within a porous polymer to the biocatalyst. This paper shows that such accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. The effect appears to be due to better swelling of the polymer in buffer and to electrostatic interactions between polymer and enzyme. (C) 2003 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)589-594
Number of pages6
Issue number3
Publication statusPublished - 12 Jan 2004


  • permanently charged groups
  • PEGA resins
  • improved biotransformations
  • solid support

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