Mucosal and systemic immune responses following mucosal immunisation of tetanus toxoid entrapped in lipid nanoparticles prepared by microwave reactor

Ayman Gebril; Mohammad A. Obeid; Ewan M. Bennett; Anairis Pujol; Mario L. Chovel; Tatiana Mahy; Reinaldo Acevedo; Valerie A. Ferro

doi:10.1016/j.ejpb.2021.12.014

Mucosal and systemic immune responses following mucosal immunisation of tetanus toxoid entrapped in lipid nanoparticles prepared by microwave reactor

Ayman Gebril, Mohammad A. Obeid, Ewan M. Bennett, Anairis Pujol, Mario L. Chovel, Tatiana Mahy, Reinaldo Acevedo, Valerie A. Ferro

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, the use of a microwave reactor, which allowed high input of energy into a pressurised system in a short period of time, was investigated for preparation of lipid nanoparticles (LNPs). The aim was to optimise the formulation process by reducing manufacturing time. Two types of LNPs were prepared; non-ionic surfactant vesicles (NISV) and bilosomes (modified NISV incorporating bile salts), with a model antigen (tetanus toxoid, TT) and the immune response induced after mucosal (nasal and oral, respectively) administration was assessed. The TT loaded LNPs were characterised in terms of particle size, size distribution, morphology, and entrapment efficiency. Immunisation was evaluated by lethal challenge with tetanus toxin in an animal model. The efficiency of vaccination was evaluated by measuring the anti-TT IgG antibody levels in the vaccinated animals. Bilosomes formed by this method showed an immunogen entrapment efficiency of ∼ 30% which was significantly (p

Original language	English
Pages (from-to)	11-18
Number of pages	8
Journal	European Journal of Pharmaceutics and Biopharmaceutics
Volume	171
Early online date	4 Jan 2022
DOIs	https://doi.org/10.1016/j.ejpb.2021.12.014
Publication status	Published - 28 Feb 2022

Keywords

microwave reactor
niosomes
mucosal immunisation
and bilosomes
tetanus toxin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ejpb.2021.12.014

Embargoed Document

Gebril-etal-EJPB-2022-Mucosal-and-systemic-immune-responses-following-mucosal-immunisation
Accepted author manuscript, 881 KB
Licence: CC BY-NC-ND 4.0
Embargo ends: 4/01/23

Cite this

Gebril, A., Obeid, M. A., Bennett, E. M., Pujol, A., Chovel, M. L., Mahy, T., Acevedo, R., & Ferro, V. A. (2022). Mucosal and systemic immune responses following mucosal immunisation of tetanus toxoid entrapped in lipid nanoparticles prepared by microwave reactor. European Journal of Pharmaceutics and Biopharmaceutics, 171, 11-18. https://doi.org/10.1016/j.ejpb.2021.12.014

Gebril, Ayman ; Obeid, Mohammad A. ; Bennett, Ewan M. ; Pujol, Anairis ; Chovel, Mario L. ; Mahy, Tatiana ; Acevedo, Reinaldo ; Ferro, Valerie A. / Mucosal and systemic immune responses following mucosal immunisation of tetanus toxoid entrapped in lipid nanoparticles prepared by microwave reactor. In: European Journal of Pharmaceutics and Biopharmaceutics. 2022 ; Vol. 171. pp. 11-18.

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Mucosal and systemic immune responses following mucosal immunisation of tetanus toxoid entrapped in lipid nanoparticles prepared by microwave reactor. / Gebril, Ayman; Obeid, Mohammad A.; Bennett, Ewan M.; Pujol, Anairis; Chovel, Mario L.; Mahy, Tatiana; Acevedo, Reinaldo; Ferro, Valerie A.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 171, 28.02.2022, p. 11-18.

Research output: Contribution to journal › Article › peer-review

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