Practical implementation of adaptive optics in multiphoton microscopy

P.N. Marsh, D. Burns, J.M. Girkin

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212 Citations (Scopus)


A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, x40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 µm with a resolution defined by a FWHM axial point-spread function of 1.25 µm.
Original languageEnglish
Pages (from-to)1123-1130
Number of pages7
JournalOptics Express
Issue number10
Publication statusPublished - 2003


  • optics
  • photonics
  • lasers
  • quantum electronics

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