Protease-catalyzed peptide synthesis on solid support

R.V. Ulijn, B. Baragana, P.J. Halling, S.L. Flitsch

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91 Citations (Scopus)


The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.
Original languageEnglish
Pages (from-to)10988-10989
Number of pages1
JournalJournal of the American Chemical Society
Issue number37
Publication statusPublished - 18 Sep 2002


  • Soluble polymeric support
  • safety catch linker
  • enzymatic- synthesis
  • organic-solvents
  • biocatalysis
  • enzymes
  • phase
  • chemistry
  • resin

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