RNA stability in frozen/thawed clinical blood samples

Olga Maria Posada Estefan; Rothwelle Tate; Mary Grant

RNA stability in frozen/thawed clinical blood samples

Olga Maria Posada Estefan, Rothwelle Tate, Mary Grant

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to conference › Abstract › peer-review

Abstract

The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial
we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction.

Original language	English
Pages	9
Number of pages	1
Publication status	Published - 2011
Event	British Toxicology Society Meeting - Nottingham, United Kingdom Duration: 5 Sep 2011 → 6 Sep 2011

Conference

Conference	British Toxicology Society Meeting
Country/Territory	United Kingdom
City	Nottingham
Period	5/09/11 → 6/09/11

Keywords

genes
blood cells
RNA stability
gene expression analysis

Cite this

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title = "RNA stability in frozen/thawed clinical blood samples",

abstract = "The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction. ",

keywords = "genes, blood cells, RNA stability , gene expression analysis",

author = "{Posada Estefan}, {Olga Maria} and Rothwelle Tate and Mary Grant",

year = "2011",

language = "English",

pages = "9",

note = "British Toxicology Society Meeting ; Conference date: 05-09-2011 Through 06-09-2011",

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TY - CONF

T1 - RNA stability in frozen/thawed clinical blood samples

AU - Posada Estefan, Olga Maria

AU - Tate, Rothwelle

AU - Grant, Mary

PY - 2011

Y1 - 2011

N2 - The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction.

AB - The main challenge of RNA extraction from frozen blood samples is to obtain sufficient RNA of acceptable quality for gene expression analysis. In this trial we compared the RNA stability in clinical blood samples with and without a RNA stabilizer, RNAlater. Results show that high quality RNA can be extracted from blood with RNAlater that has been frozen and thawed several times before the extraction.

KW - genes

KW - blood cells

KW - RNA stability

KW - gene expression analysis

UR - http://www.robots.ox.ac.uk/~davidc/pubs/pgbiomed_proc2011.pdf

M3 - Abstract

SP - 9

T2 - British Toxicology Society Meeting

Y2 - 5 September 2011 through 6 September 2011

ER -

RNA stability in frozen/thawed clinical blood samples

Abstract

Conference

Keywords

Other files and links

Cite this