Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS

A.M. Ingram, B.D. Moore, D. Graham

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Surface enhanced resonance Raman scattering (SERRS) is an alternative to fluorescence for use in bioanalysis however due to the different optical mechanism it requires specifically designed reporters. Recently we have reported the use of 8-hydroxyquinolinyl azo dyes and their ester derivatives as reporters of lipase activity using SERRS. Acylation of the 8-hydroxy moiety significantly reduces surface enhancement of the Raman response and subsequent lipase catalysed ester hydrolysis enables the analyte to bind to silver nanoparticles, thus providing surface enhancement and the SERRS signal is 'switched on'. By following this principle, phosphorylated and galactosylated analogues of 8-hydroxyquinolinylazo dyes were prepared and shown to act as reporters of enzymatic activity for alkaline phosphatase and β-galactosidase respectively when using SERRS.
Original languageEnglish
Pages (from-to)1569-1571
Number of pages2
JournalBioorganic and Medicinal Chemistry Letters
Issue number6
Publication statusPublished - 15 Mar 2009


  • enzymes
  • surface enhanced Raman scattering

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