Spherical molecularly imprinted polymer particles: A promising tool for molecular recognition in capillary electrokinetic separations

T. de Boer, R. Mol, R.A. de Zeeuw, G.J. de Jong, D.C. Sherrington, P.A.G. Cormack, K. Ensing

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Spherical molecularly imprinted polymer particles obtained via precipitation polymerization, were introduced as a pseudostationary phase in capillary electrophoresis (CE) to study molecular recognition. Analyses were performed via a partial filling technique using (+)-ephedrine-imprinted microspheres (100-200 nm) which were polymerized from methacrylic acid and 1,1,1-Tris(hydroxymethyl)propanetrimethacrylate using acetonitrile as the solvent. The influence of pH and the modifier content on the separation was investigated. A 0.1 % w/v suspension in an aqueous 10 mm phosphate buffer (pH 2.5 with 40% acetonitrile) was hydrodynamically injected into the CE system (80% of the effective capillary length) and led to full baseline separation of racemic ephedrine within 10 min.
Original languageEnglish
Pages (from-to)1296-1300
Number of pages4
Issue number9
Publication statusPublished - May 2002


  • capillary electrokinetic chromatography
  • molecular recognition
  • molecularly imprinted polymers

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