Substance P: endothelial localization and pharmacology in the human ovarian vein

R.W. Stones, A. Loesch, R.W. Beard, G. Burnstock

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To investigate the possible role of substance P as an endothelial factor in the local regulation of vascular tone in the human ovarian vein.

We performed immunolocalization of substance P in human ovarian venous endothelium in situ and in culture, and observed responses to substance P in preconstricted ring preparations of human ovarian vein in the presence of either the prostaglandin synthesis inhibitor indomethacin or the inhibitor of nitric oxide synthesis l-nitro arginine methyl ester (l-NAME), with and without luminal rubbing.

Substance P was localized in a subpopulation of ovarian vein endothelial cells. Maximal relaxation induced by substance P was not significantly affected by indomethacin (10 μmol/L), but was reduced from 58.7% (95% confidence interval [CI] 41.3–76.1) in control experiments to 24.7% (95% CI 18.3–31.1) after luminal rubbing and to 32.3% (95% CI 19.8–44.8) after exposure to l-NAME (0.1 mmol/L) (P = .001).

The localization of substance P in ovarian vein endothelium together with vasodilator effects mediated partially via the endothelium suggests that the peptide has a role in the local control of vascular tone in this vessel. (Obstet Gynecol 1995;85:273–8)
Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalObstetrics & Gynecology
Issue number2
Publication statusPublished - 28 Feb 1995


  • adult
  • arginine
  • carboprost
  • dose-response relationship
  • drug
  • endothelium
  • vascular
  • female
  • humans
  • immunohistochemistry
  • indomethacin
  • middle aged
  • NG-nitroarginine methyl ester
  • nitric oxide
  • ovary
  • substance P
  • vasodilation
  • veins

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