Using tablet for visual exploration of second-generation sequencing data

Iain Milne, Gordon Stephen, Micha Bayer, Peter J.A. Cock, Leighton Pritchard, Linda Cardle, Paul D. Shawand, David Marshall

Research output: Contribution to journalArticlepeer-review

524 Citations (Scopus)


The advent of second-generation sequencing (2GS) has provided a range of significant new challenges for the visualization of sequence assemblies. These include the large volume of data being generated, short-read lengths and different data types and data formats associated with the diversity of new sequencing technologies. This article illustrates how Tabletça high-performance graphical viewer for visualization of 2GS assemblies and read mappingsçplays an important role in the analysis of these data. We present Tablet, and through a selection of use cases, demonstrate its value in quality assurance and scientific discovery, through features such as whole-reference coverage overviews, variant highlighting, paired-end read mark-up, GFF3-based feature tracks and protein translations. We discuss the computing and visualization techniques utilized to provide a rich and responsive graphical environment that enables users to view a range of file formats with ease. Tablet installers can be freely downloaded from in 32 or 64-bit versions for Windows, OS X, Linux or Solaris. For further details on theTablet, contact

Original languageEnglish
Pages (from-to)193-202
Number of pages10
JournalBriefings in Bioinformatics
Issue number2
Publication statusPublished - 31 Mar 2013


  • assembly validation
  • second-generation sequencing
  • visualization

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