Effects of L-glutamate and ADP on immunomodulation of microglia, relevance to stroke

  • Darren Eskdale

Student thesis: Master's Thesis

Abstract

Background: Microglia are resident glial cells of the CNS, with potent immunomodulatory effects in stroke. Their defined split nature of both pro-inflammatory and anti-inflammatory paves research to define their behaviour under a variety of conditions. ADP and L-glutamate are abundant in the extracellular fluid following brain insults such as stroke and therefore they may represent influential mediators in controlling microglial cell function. Methods: In vitro, exogenous ADP/L-glutamate (50μg/ml) was applied to BV-2 microglia cells and key aspects of cell function were measured by Western blotting, including p-ERK and p.p38, proteins involved in a cascade of events, as well as iNOS and arginase 1 to assess microglial phenotype. NO release was assessed by Griess assay and chemotactic behaviour was analysed using microfluidics and time lapse imaging. In addition, the effects of pre-conditioning with ADP on microglia was assessed by measuring iNOS expression in response to LPS (1μg/ml) stimulation.Results: ADP significantly increased (p
Date of Award17 Mar 2017
Original languageEnglish
Awarding Institution
  • University Of Strathclyde
SupervisorHilary Carswell (Supervisor) & Michele Zagnoni (Supervisor)

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